Pet Safety / Compounds / Milbemycin oxime

Is Milbemycin oxime safe for dogs and cats?

Moderate risk for pets

Milbemycin oxime presents a low to moderate risk to dogs depending on breed and MDR1/ABCB1 genetic status. In MDR1 wild-type dogs (the majority of dog breeds), milbemycin oxime at FDA-approved doses (0.5–1 mg/kg monthly) has an excellent safety margin for heartworm prevention and parasite control. The primary safety concern is neurotoxicity in MDR1-deficient dogs (Rough/Smooth Collies: ~70% carrier rate; Australian Shepherds, Shetland Sheepdogs, Border Collies, and related herding breeds: variable carrier rates of 10–50%). MDR1 −/− homozygous dogs are at high risk of severe neurological toxicity (ataxia, tremors, coma) even at approved doses. MDR1 +/− heterozygotes have intermediate susceptibility. Veterinary prescribers should: (1) perform MDR1 genetic testing for susceptible breeds before prescribing macrolide antiparasitics; (2) use alternative antiparasitic classes if MDR1 −/− status is confirmed. Concurrent use with drugs that inhibit P-glycoprotein (e.g., ketoconazole, cyclosporine) can increase CNS milbemycin levels even in MDR1 wild-type dogs.

What is milbemycin oxime?

The IUPAC name is (1R,4S,5'S,6R,6'R,8R,10E,13R,14E,16E,20R,21Z,24S)-6'-ethyl-24-hydroxy-21-hydroxyimino-5',11,13,22-tetramethylspiro[3,7,19-trioxatetracyclo[15.6.1.14,8.020,24]pentacosa-10,14,16,22-tetraene-6,2'-oxane]-2-one;(1R,4S,5'S,6R,6'R,8R,10E,13R,14E,16E,20R,21Z,24S)-24-hydroxy-21-hydroxyimino-5',6',11,13,22-pentamethylspiro[3,7,19-trioxatetracyclo[15.6.1.14,8.020,24]pentacosa-10,14,16,22-tetraene-6,2'-oxane]-2-one.

Also known as: (1R,4S,5'S,6R,6'R,8R,10E,13R,14E,16E,20R,21Z,24S)-6'-ethyl-24-hydroxy-21-hydroxyimino-5',11,13,22-tetramethylspiro[3,7,19-trioxatetracyclo[15.6.1.14,8.020,24]pentacosa-10,14,16,22-tetraene-6,2'-oxane]-2-one;(1R,4S,5'S,6R,6'R,8R,10E,13R,14E,16E,20R,21Z,24S)-24-hydroxy-21-hydroxyimino-5',6',11,13,22-pentamethylspiro[3,7,19-trioxatetracyclo[15.6.1.14,8.020,24]pentacosa-10,14,16,22-tetraene-6,2'-oxane]-2-one, Interceptor flavor tabs, Milbemite, CGA-179246.

IUPAC name
(1R,4S,5'S,6R,6'R,8R,10E,13R,14E,16E,20R,21Z,24S)-6'-ethyl-24-hydroxy-21-hydroxyimino-5',11,13,22-tetramethylspiro[3,7,19-trioxatetracyclo[15.6.1.14,8.020,24]pentacosa-10,14,16,22-tetraene-6,2'-oxane]-2-one;(1R,4S,5'S,6R,6'R,8R,10E,13R,14E,16E,20R,21Z,24S)-24-hydroxy-21-hydroxyimino-5',6',11,13,22-pentamethylspiro[3,7,19-trioxatetracyclo[15.6.1.14,8.020,24]pentacosa-10,14,16,22-tetraene-6,2'-oxane]-2-one
CAS number
129496-10-2
Molecular formula
C63H88N2O14
Molecular weight
1097.4 g/mol
SMILES
CCC1C(CCC2(O1)CC3CC(O2)CC=C(CC(C=CC=C4COC5C4(C(C=C(C5=NO)C)C(=O)O3)O)C)C)C.CC1CCC2(CC3CC(O2)CC=C(CC(C=CC=C4COC5C4(C(C=C(C5=NO)C)C(=O)O3)O)C)C)OC1C
PubChem CID
20056431

Risk for dogs

Moderate risk

Milbemycin oxime presents a low to moderate risk to dogs depending on breed and MDR1/ABCB1 genetic status. In MDR1 wild-type dogs (the majority of dog breeds), milbemycin oxime at FDA-approved doses (0.5–1 mg/kg monthly) has an excellent safety margin for heartworm prevention and parasite control. The primary safety concern is neurotoxicity in MDR1-deficient dogs (Rough/Smooth Collies: ~70% carrier rate; Australian Shepherds, Shetland Sheepdogs, Border Collies, and related herding breeds: variable carrier rates of 10–50%). MDR1 −/− homozygous dogs are at high risk of severe neurological toxicity (ataxia, tremors, coma) even at approved doses. MDR1 +/− heterozygotes have intermediate susceptibility. Veterinary prescribers should: (1) perform MDR1 genetic testing for susceptible breeds before prescribing macrolide antiparasitics; (2) use alternative antiparasitic classes if MDR1 −/− status is confirmed. Concurrent use with drugs that inhibit P-glycoprotein (e.g., ketoconazole, cyclosporine) can increase CNS milbemycin levels even in MDR1 wild-type dogs.

Regulatory consensus

1 regulatory bodyhas classified Milbemycin oxime.

AgencyYearClassificationNotes
IARC2023Not evaluated by IARC — milbemycin oxime is an FDA/CVM-approved veterinary macrolide antiparasitic (dogs, cats) for heartworm prevention, mite treatment, and intestinal parasite control; no carcinogenicity classification; primary safety concern is neurotoxicity in MDR1 (ABCB1)-deficient dog breeds (Collies, Shetland Sheepdogs, Australian Shepherds and related breeds with P-glycoprotein deficiency)

Regulators apply different standards of evidence — animal-data weighting, exposure-pattern assumptions, epidemiological power thresholds — which is why two scientific bodies can review the same data and reach different conclusions. The disagreement is the data.

Where pets encounter milbemycin oxime

  • Industrial FacilitiesManufacturing plants, Chemical storage areas, Waste treatment sites
  • Occupational EnvironmentsFactories, Warehouses, Transportation vehicles

Safer alternatives

Lower-risk approaches that achieve a similar outcome to Milbemycin oxime:

  • Alternative drug class; Non-pharmacological therapy; Lowest effective dose
    Trade-offs: Direct chemical substitution requires verification that the replacement does not introduce new hazards (regrettable substitution). Conduct full hazard assessment of proposed alternative before adoption.
    Relative cost: 1.2-2×

Frequently asked questions

Is milbemycin oxime safe for pets?

Milbemycin oxime presents a low to moderate risk to dogs depending on breed and MDR1/ABCB1 genetic status. In MDR1 wild-type dogs (the majority of dog breeds), milbemycin oxime at FDA-approved doses (0.5–1 mg/kg monthly) has an excellent safety margin for heartworm prevention and parasite control. The primary safety concern is neurotoxicity in MDR1-deficient dogs (Rough/Smooth Collies: ~70% carrier rate; Australian Shepherds, Shetland Sheepdogs, Border Collies, and related herding breeds: variable carrier rates of 10–50%). MDR1 −/− homozygous dogs are at high risk of severe neurological toxicity (ataxia, tremors, coma) even at approved doses. MDR1 +/− heterozygotes have intermediate susceptibility. Veterinary prescribers should: (1) perform MDR1 genetic testing for susceptible breeds before prescribing macrolide antiparasitics; (2) use alternative antiparasitic classes if MDR1 −/− status is confirmed. Concurrent use with drugs that inhibit P-glycoprotein (e.g., ketoconazole, cyclosporine) can increase CNS milbemycin levels even in MDR1 wild-type dogs.

What products contain milbemycin oxime?

Milbemycin oxime appears in: Manufacturing plants (Industrial facilities); Chemical storage areas (Industrial facilities); Factories (Occupational environments); Warehouses (Occupational environments).

See Milbemycin oxime in the pets app

Look up products containing milbemycin oxime, compare to alternatives, and explore the full data record.

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Sources (1)

  1. Milbemycin Oxime FDA CVM Interceptor Sentinel Trifexis; Streptomyces hygroscopicus Macrolide Milbemycin A3 A4 Oxime; GluCl GABA-Gated Chloride Channel Invertebrate; MDR1 ABCB1 P-Glycoprotein Collie Herding Breed Neurotoxicity; Dirofilaria Heartworm Prevention; Demodex Sarcoptes Mange; WSU MDR1 Genetic Testing; Log Kow Aquatic Invertebrate Ecotoxicity; IARC Not Evaluated (2023) — regulatory

Reference data, not professional advice. Aggregates publicly available regulatory and scientific data; not a substitute for veterinary, medical, legal, or regulatory advice. Why we built ALETHEIA →